Diluentes contendo extratos vegetais para a criopreservação de sêmen de jundiá (Rhamdia quelen)

Abstract

The aim of the present study was to maximize the use of cryopreserved semen of the species Rhamdia quelen collected from the reproductive peak and outside the breeding season, using different extracts of Eichornia crassipes and curcumin in the diluting solution, in order to assess the viability and yield of cryopreserved sperm. Thirty males (130.00 ± 3.47g) were used in the first trial and 20 males (153 ± 0.04g) in the second trial. The induced semen was collected, evaluated and homogenized to form composite samples. Then the semen was diluted in a solution containing 5% fructose, 5% powdered milk, 10% methanol, in the same diluent five different extracts were added: 0.75% ERA = aqueous root extract, 0.5% EAA = aerial aqueous extract, 0.5% EAFP = aqueous polar fraction extract (first test). The same procedure was applied in the second trial using 1% and 1.5% ERH = hydromethanolic extract of the root; 0.75% and 1% ERA = aqueous root extract; 0.5% and 1% ERAq = heated root extract; 1.25% and 1.5% EAH = aerial hydromethanolic extract; 0.5% and 1% EAA = aerial aqueous extract; 0.5% and 1% EAAq = heated aerial extract. After 30 days, the semen was thawed to evaluate the sperm movement, sperm quality, biochemistry and cryopreserved sperm yield. For fertilization, 20 females of R. quelen (191.00 ± 0.05g) were used in the first trial and 25 females (202.00 ± 0.09g) in the second trial. Then, the females were induced to ovulate and the oocytes were collected and fertilized using cryopreserved semen with the respective diluents. Fertilization, hatching and larval normality rates were evaluated. Diluents containing E. crassipes extracts improved (p <0.05) motility compared to the standard diluent in the two trials. They also improved (p <0.05) membrane morphology and integrity, using extracts of E. crassipes and curcumin. In the second trial, there was a reduction in lipid peroxidation using E. crassipes extracts. The effective yield and the effective dose were also improved with the use of E. crassipes extracts. Therefore, it is concluded that diluents containing extracts of E. crassipes and curcumin guarantee protection of sperm during cryopreservation, increase cell viability. In addition to improving semen yield outside the breeding season.