Produção de monoglicerídeos e diglicerídeos a partir da reação de glicerólise catalisada por lipase imobilizada

Abstract

The present work aimed to monitor the production of monoacylglycerols (MAG) and diacylglycerols (DAG) from olive oil, using Tween 80 as surfactant, butanol as co-surfactant and Lipozyme TL 100L enzyme as biocatalyst, immobilized on support Spectrogel type C to obtain emulsifying agents with higher added value. The stability of the micros that had stability to glycerolysis that presented greater efficiency of conversion of triacyllons (TAG) was carried out. The points for a glycerolysis were submitted to factorial planning 22. This methodology was adopted for the production of DAG, and these conversion factors of approximately 95% of TAG in MAG and DAG were found. In order to optimize the methodology, with the help of the Box-Behnken mathematical model, a mathematical model was elaborated with the planning results and a theoretical value of 97% of performance with the use of 7.95% (m/m) of enzyme at 35.97°C. For this, the theoretical and experimental points were reproduced, and the optimum point was obtained under the conditions of glycerol: oil molar ratio 2:1, 36ºC, 7.95% (m/m) of enzyme and in a reaction time of reaction 120 minutes, and a conversion efficiency of approximately 97% in MAG and DAG. The adsorption was optimized in the same enzymatic way as the glycerolysis conditions and used as optimal for the adsorption process, obtaining a theoretical maximum of 96 U g-1 of activity or practical 93 U g-1 of average value. Therefore, the experimental results had similar results when compared with theorical results, and such conditions were established in the conversion. Enzyme activity is also compatible with the same parameter, or increased by applying the theoretical point.