Vitaminas C e E como aditivos em soluções crioprotetoras empregadas no congelamento de sêmen do Rhamdia quelen

Abstract

This study was conducted to evaluate the effect of the addition of vitamins C and E in cryoprotectant solutions used in freezing semen of Rhamdia quelen on the parameters of sperm motility after thawing. Assays were conducted: (1) employment of 0.0, 4.0, 6.5, 9.0 and 11.5 mg of vitamin C (monovin C ®). ML-1, (2) employment of 0.0, 2.0, 4.0, 6.0 and 8.0 mg of vitamin E (monovin E ®), (3) employment of 0.0, 4.0 +2.0, 6.5 + 4.0, 9.0 +6.0 and 11.5 +8.0 mg vitamin C (monovin ® C) + mg of vitamin E (monovin E ®.) mL-1 respectively. Procedures for cryopreservation, the semen was diluted in cryoprotectant solution at a ratio of 1:3, subjected to pre freezing in nitrogen vapor and then plunged into liquid nitrogen. This material was thawed by immersion in water at room temperature for about eight seconds. The rate of sperm parameters of sperm motility (MOT), curvilinear velocity (VCL), average velocity of displacement (VMD) and straight-line speed (VLR), semen fresh and thawed semen were measured by computational analysis (CASA) . It was found that the use of only vitamin C levels between 4.0 and 6.5 showed better results (P <0.05) related to sperm motility of cells, which presented only with the use of vitamin E, which had a deleterious effect on sperm cells (p = 0.0086 for linear speed) and the combined use of both vitamins (C + E) showed significant value (p = 0.0029) for sperm motility, a fact however, that no should be considered, because the values of average speed (p = 0.0149) and linear velocity (p = 0.0006) demonstrated a deleterious effect on sperm cells.