Estudo histopatológico em juvenis de Rhamdia quelen submetidos a ensaio toxicológico com ibuprofeno

Abstract

The toxicological test was performed to determine the toxic potential of the drug ibuprofen through histopathological eyepieces in gills and livers of Rhamdia quelen juveniles submitted to different concentrations of Ibuprofen (0,0; 0,5; 5,0 e 50,0 mg/L). These were determined from acute tests with an CL50 of 5,0mg/L. For each of them, four replicates, with four specimens each. The experiment lasted 120 hours. In preparing the material for the routine histological technique for inclusion in paraffin, and as analyzes as slides were stained with Hematoxylin and Eosin. Our analogues were tested, such as: Incomplete lamellae and aneurysm fusion, among others, for gills and vacuolization, cytoplasmic and focal necrosis, among others, for livers, which were classified in stages I, II and III for Severity of injuries, determined the Index of Historical Changes and Average Values of Changes. The drug presented toxic potential for this species, even at low concentrations, and damages ranging from moderate to severe in the livers and gills of the fish analyzed were observed.