Atividade de Micocinas produzidas por Wickermomyces anomalus incorporada em um gel dermatológico frente á Cutibacteroum acnes

Abstract

Acne is a chronic inflammatory skin disorder influenced by multiple factors, including hormonal changes, skin type characteristics, and genetic predisposition. Cutibacterium acnes is a Gram-positive, facultative anaerobic, non-spore-forming bacterium that resides on human skin. While some strains are commensal, others act as opportunistic pathogens, contributing to invasive skin infections and the development of acne. Mycocins, natural substances produced by killer yeasts, exhibit broad-spectrum antimicrobial activity against bacteria, filamentous fungi, and yeasts, with low toxicity and reduced likelihood of microbial resistance. Wickerhamomyces anomalus is recognized as a potent producer of mycocins with significant industrial application potential. The aim of this study was to evaluate the antimicrobial activity of mycocins from W. anomalus against C. acnes and to develop a dermatological gel incorporating these compounds for acne treatment. Antimicrobial assays were performed using solid media and microdilution methods, followed by preliminary gel stability tests and shelf-life evaluation. Results demonstrated that C. acnes growth was inhibited in solid medium. In microdilution assays, C. acnes were sensitive to mycocins present in the culture supernatant of W. anomalus at a minimum inhibitory concentration of 0.067 U/mg. Gel formulations containing mycocins remained stable under different storage conditions. In summary, the findings confirmed the antimicrobial activity of mycocins produced by W. anomalus in inhibiting C. acnes growth, as well as the stability of the gel formulation, highlighting its potential as a novel dermatological product for acne management.