Utilização do permeado de soro de leite na produção de celulases por fermentação em estado sólido do bagaço de cana

Abstract

The great market potential and the important role that cellulases impact on bioenergy and organic products industries cause a great motivation in the development of improved cellulolytic enzymes diming to the hydrolysis of cellulose of plant cell walls. These cellulases must have some necessary characteristics for application in biorefineries as high catalytic efficiency of insoluble cellulosic substrates. The objective of this study was to evaluate the feasibility of using whey permeate, in the culture medium composition for the enzymatic complex production of cellulases, such as FPases, CMCases, xylanase and β-glucosidase by solid state fermentation of bagasse sugarcane using the fungus Aspergillus niger. It was evalusted the optimize the production process of cellulolytic enzymes by SSF, evaluating the influence of pH and concentration of whey permeate the culture medium. Studies were also performed on the enzymatic hydrolysis of sugarcane bagasse by an experimental design. Regarding the enzymatic production it showed good results for FPase enzymes, CMCase and β-glucosidase. For FPase there was a production of 37,0 U.gms-1. For the xylanase there was a production of 2,6 U.gms-1.For CMCase there was a production of 39,0 U.gms-1. For β-glucosidase there was a production of 18.0 U.gms-1 in 48 h of fermentation. In time the production kinetics, it used the optimal conditions of fermentation solid-state 60 g.L-1 PSL and pH 5.0 for FPase enzymes, CMCase and β-glucosidase. This study indicated that the fungus Aspergillus niger showed considerable amount of enzymes cellulolytic complex except the xylanase enzyme, stimulating the continuity of study for other enzymes to alter the nutritional conditions of cultivation to increase production of cellulases.