Avaliação do uso de fibra obtida por eletrofiação contendo óleo de Girassol ozonizado na cicatrização de lesões estéreis

Abstract

Ozone (O3) is a highly reactive inorganic molecule; that is part of the atmosphere and can also be produced by ozone generators. It is a potent microbicidal agent, having antibacterial, antiviral, antifungal, and antiparasitic properties, which can have its mechanism of action divided into three aspects: immunomodulation, antimicrobial activity, and ability to interact with the oxidant/antioxidant balance. In this regard, ozonated oil has been used topically to treat chronic wounds. The electrospinning technique (or electrostatic spinning) has the potential to produce materials made of nanofibers, and these nanofibers, when assembled, can give rise to blankets and membranes, presenting a large specific surface area, high porosity, and interconnectivity, making them suitable for the nutrient transport, cellular communication, and efficient cellular responses. Wound healing is a multiphase process consisting of three overlapping stages: inflammation, tissue proliferation, and remodeling. Adjuvant therapy is often required because of low healing rates in chronic wounds treated with standard care. Therefore, this work aims to verify the efficiency of ozonized Sunflower oil incorporated into electrospun fibers as a healing method in excisional lesions on the back of BALB/c mice. The electrospun fiber (Ecovio®) showed great promise in reducing the lesion, especially in the initial days (3 days). It was also able to significantly reduce the number of leukocytes in 7 days, showing its anti-inflammatory effect. Sunflower oil, whether ozonated or not, appears to bring greater hydration to the lesion, but further research is needed with different concentrations of ozone in the oil, as there have been controversial results.