Análise Histológica do Tecido Adiposo Branco de roedores obesos tratados com Óleo de Castanha do Brasil.

Abstract

Adipocyte hypertrophy is related to worsen metabolic condition in obese state. Bioactive compounds present in natural products may have health benefits, including improving obesity-related comorbidities. In this study, it was evaluated the effect of chronic supplementation with Brazil nut oil (Bno) on the frequency of distribution of adipocytes in the visceral and subcutaneous white adipose tissue of obese rats. Hypothalamic obesity was induced in males (♂) and female (♀) rats by neonatal glutamate monosodium (MSG) administration (4g/Kg). Control (CTL; ♂ and ♀) received equimolar saline solution. From 30 – 90 days of life half MSG and CTL groups received by gavage via orogastric the Bno a dose of 1mL/Kg. Non supplemented (NS) groups received same volume of saline (0.9%). At 92 days of life were registered body weight (BW), naso-anal length (NAL) and calculated Lee index. White adipose tissue depots from inguinal (WAT-I) and perirenal (WAT-P) regions were removed, weighed and submitted to histological procedures. Adipocyte size and distribution frequency (small, medium, and large) were evaluated in obese and non obese WAT-I and WAT-P deposits, ♂ and ♀). MSG ♂ and ♀ rodents showed increased WAT-I and WAT-P content and adipocyte hypertrophy compared to non obese animals (CTL). In addition, a high frequency of large adipocytes and a simultaneous reduction in the frequency of small adipocytes were observed in WAT-I and WAT-P deposits of MSG-treated ♂ and ♀ compared to the respective CTL groups. Bno supplementation significantly reduced adipocyte hypertrophy in WAT-P in obese groups (MSGbno ♂ and ♀) with a simultaneous increase in the frequency of small and medium adipocytes compared to MSGNS ♂ and ♀ rats. These effects were more evident in obese females. In this group, supplementation with Bno also improved the adipocyte profile of WAT-I. Thus, we conclude that chronic supplementation of Bno is effective in reducing adipocyte hypertrophy in WAT-P of hypothalamic obese animals, exerting more pronounced effects on the in obese females.