Isolamento e seleção de leveduras de ambiente continental lótico com capacidade de produção enzimática

Abstract

Yeasts have wide applicability in the industrial scope, one of these being is through enzymatic synthesis. Biocatalysts are more efficient when compared to chemical catalysts, especially amylase, cellulase, protease and lipase. The types of fermentation used to produce of enzymes are submerged fermentation and solid state fermentation. After production, enzymes and cells can be used in free form. The objective of this study was to prospect yeasts with biotechnological importance, isolated in the water of the Toledo River, Toledo-PR, with the capacity to synthesize hydrolases and to analyze the lipase quantitatively. 75 microorganisms were isolated in YPD + antibacterial medium and their capacity for enzymatic production was tested in specific medium. The best producers were subjected to molecular analyzes and solid state fermentation (FES) techniques to quantify the enzyme lipase. Of the isolates 8 presented good enzyme indexes. The yeast Candida sp. JC054, used in the fermentation test for the lipase production, presented an enzymatic peak of 309.6 U in 120 hours. The results showed that the isolated yeasts are good enzymatic producers, with prominence for Candida sp., which is promising in obtaining lipase via FES.