https://tede.unioeste.br/handle/tede/586 Tue, 03 Feb 2026 20:45:21 GMT 2026-02-03T20:45:21Z https://tede.unioeste.br/handle/tede/8213 Title: Educação do Campo e princípios éticos da permacultura: um projeto de escola visando a formação do sujeito ecológico Autor: Arcanjo, Marilda Candido dos Santos Primeiro orientador: Bona, Celito De Abstract: This dissertation aims to investigate how the integration between Rural Education and the ethical principles of permaculture, caring for the Earth, Caring for People, and Sharing Surpluses, can contribute to the construction of a pedagogical project focused on the formation of the ecological subject. The research starts from the premise that the rural school, by valuing traditional knowledge, community life, and the relationship with the territory, constitutes a privileged space for the development of critical, contextualized, and transformative educational practices. Among the specific objectives, the following stand out: identifying the environmental content present in Brazilian public schools; conceptualizing the terms "place" and "rural situation" in light of environmental literature; discussing the theoretical and practical challenges of socio-environmental education within the scope of Rural Education; The research, with a qualitative and exploratory approach, is based on a literature review and a study of projects carried out in school institutions where the results were satisfactory. Thus, the analysis of educational experiences revealed that permaculture, when incorporated into the school curriculum as an ethical and methodological foundation, expands the possibilities for holistic education, promoting autonomy, belonging, and ecological awareness among students. As a result of the study, it is concluded that the articulation between Rural Education and permaculture offers a viable and promising path for the formation of the ecological subject, a critical individual rooted in their territory and committed to environmental regeneration and social transformation. The proposal presented contributes to the strengthening of sustainable pedagogical practices, aligned with the Sustainable Development Goals (SDGs), and reaffirms the role of the rural school as a space of resistance, innovation, and emancipation. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Tue, 12 Aug 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8213 2025-08-12T00:00:00Z https://tede.unioeste.br/handle/tede/8212 Title: Bioprospecção de compostos com atividade antimicrobiana em méis de Scaptotrigona bipunctata Autor: Pezenti, Leticia Primeiro orientador: Eising, Renato Abstract: The increasing threat of antimicrobial resistance (AMR) has intensified the search for new bioactive compounds from natural sources. In this context, stingless bees and their products represent promising alternatives due to their rich microbial symbioses. This study investigated the antimicrobial activity of honeys produced by Scaptotrigona bipunctata, a stingless bee species endemic to Brazil, and aimed to isolate microorganisms potentially responsible for this activity. Honey samples were collected from five different localities in Toledo, Paraná, Brazil. Antimicrobial susceptibility was evaluated against Staphylococcus aureus, Escherichia coli, Bacillus cereus, and Streptococcus pyogenes using agar well diffusion assays. All samples inhibited S. aureus, while no activity was observed against E. coli. The most active sample also inhibited B. cereus and S. pyogenes. A total of 25 bacterial isolates were obtained from the honeys under aerobic and anaerobic conditions. Two isolates exhibited antagonistic activity against S. aureus, with their cell-free supernatants (CFS) producing inhibition zones and showing minimum inhibitory concentrations (MICs) of 25%. The isolates were initially identified as Gram-positive bacteria. These findings indicate that S. bipunctata honey and its associated microbiota are potential sources of antimicrobial compounds, particularly against Gram-positive bacteria, underscoring their biotechnological relevance for the development of novel therapeutic agents. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Fri, 29 Aug 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8212 2025-08-29T00:00:00Z https://tede.unioeste.br/handle/tede/8126 Title: Gestão de resíduos químicos em universidades do Paraná: tratamento interno vs terceirizado Autor: Ikuno, Jeruza Wilezilek Primeiro orientador: Dragunski, Josiane Caetano Abstract: The significant increase in waste production and the need for integrated and conscious management raise questions that must necessarily begin at academic centers. As professional educators, higher education institutions in Paraná need to implement measures to address the issue of waste generation and disposal in accordance with existing legal provisions. The objective of this study is to investigate the issues of chemical waste management produced in the chemistry laboratories of Paraná's universities, focusing on a comparative analysis between in-house and outsourced waste management. The research aims to identify the main challenges, costs, benefits, and risks associated with each management model, considering current environmental legislation, the specificities of waste generated in the academic context, and the institutions' infrastructure and human resources capabilities. Through a methodological approach that combines literature review, document analysis, and technical opinions from universities, the dissertation aims to provide support for strategic decision-making regarding the most efficient, safe, and sustainable way to manage university chemical waste in the state of Paraná. The analysis considered aspects such as the complexity of the legislation, the variety of waste generated, operational costs, the technical expertise required, the risks of accidents and contamination, and the potential for implementing minimization and treatment practices. As a result of this research, a guidance booklet was created on the legislation and sanctions applicable to Higher Education Institutions. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Thu, 04 Sep 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8126 2025-09-04T00:00:00Z https://tede.unioeste.br/handle/tede/8119 Title: Hidrolisado enzimático de penas de frango em dietas para tilápia do Nilo (Oreochromis niloticus): desempenho produtivo e atratopalatabilidade Autor: Figueiredo, Elisiane da Silva Primeiro orientador: Signor, Altevir Abstract: The use of agro-industrial by-products such as viscera, blood, skins, and feathers can be an excellent alternative to fishmeal in aquaculture. This study evaluated the effects of including enzymatically hydrolyzed chicken feathers (HEP), supplied by BRF Ingredients®, in diets for juvenile Nile tilapia (Oreochromis niloticus). The experiments were conducted at the Aquaculture Laboratory of the Aquaculture Management Study Group – GEMAq, at the Western Paraná State University (UNIOESTE), Toledo Campus, Brazil, over a 50-day period for zootechnical performance and 23 days for the attractability and palatability trial.Three isoproteic diets were formulated with 0% (control diet), 1%, and 2% HEP. For productive performance, a completely randomized design was used, with 24 polyethylene tanks and 15 juveniles averaging 9.0 ± 1.0 grams, totaling 360 fish. Feeding occurred four times a day (8 a.m., 11 a.m., 2 p.m., and 5 p.m.). Tank cleaning was performed twice daily, with a partial water exchange of 30%. Dissolved oxygen averaged 4.9 ± 0.5 mg L⁻¹; pH was 7.2 ± 0.01, measured weekly, and temperature was 28.7 ± 0.3°C, measured daily using a multiparameter probe (YSI® Professional Series). Ammonia levels were 0.29 ± 0.05 ppm; nitrite 0.4 ± 0.001 ppm; and soft water hardness were monitored every three days using a LabconTest® colorimetric kit.After the experimental period, zootechnical parameters such as weight gain, specific growth rate (SGR), apparent feed conversion ratio (FCR), hepatosomatic index (HSI), viscerosomatic index (VSI), and carcass proximate composition were analyzed. For the attractability and palatability study, 12 Nile tilapia fingerlings (O. niloticus) with an initial average weight of 7.54 ± 0.39 g and standard and total lengths of 6.18 ± 0.16 cm and 7.73 ± 0.20 cm, respectively, were used. The fish were fed six times a day (8 a.m., 10 a.m., 12 p.m., 2 p.m., 4 p.m., and 6 p.m.). To obtain results, a GoPro® 5 Black 4K Ultra HD camera was used for recording, and attractability-palatability indices (API) were measured based on behavioral parameters such as time to capture the first pellet, number of pellets consumed, rejections, and approaches.Results suggest that the diet containing 2% enzymatically hydrolyzed chicken feathers (HEP) promoted better performance in terms of weight gain and SGR, without negatively affecting physiological indices or carcass composition. Regarding attractability and palatability, positive behavioral responses were observed at specific feeding times, especially for diets containing 1% and 2% HEP, resulting in higher consumption and lower feed rejection. The inclusion of up to 2% HEP proved to be a viable alternative protein source for the formulation of efficient and sustainable diets for tilapia, contributing to the utilization of these agro-industrial by-products in aquaculture. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Fri, 27 Jun 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8119 2025-06-27T00:00:00Z