https://tede.unioeste.br/handle/tede/515 Wed, 04 Feb 2026 05:51:43 GMT 2026-02-04T05:51:43Z https://tede.unioeste.br/handle/tede/8125 Title: AÇÃO INIBITÓRIA DE MICOCINAS PRODUZIDAS POR WICKERHAMOMYCES ANOMALUS EM SALMONELLA MINNESOTA ISOLADAS DE CARNES DE FRANGO Autor: Melin, Gabriel Antonio Primeiro orientador: Gandra, Rinaldo Ferreira Abstract: The export and consumption of chicken meat in Brazil have been progressively increasing, demanding rigorous microbiological analyses to ensure food safety. Among the most relevant pathogens is Salmonella minnesota, a serovar associated with antimicrobial resistance and frequently detected in poultry products. This study aimed to evaluate the production of β-1,3-glucanases by the yeast Wickerhamomyces anomalus and its antimicrobial activity against S. minnesota strains isolated from chicken meat. A total of 73 Salmonella spp. isolates were typified, of which 27 were confirmed as S. minnesota. The antimicrobial action of the mycocins was assessed through solid media and microdilution assays, with complete inhibition of all 27 strains observed at a concentration of 0.11 U/mg of β-glucanases present in the supernatant of the W. anomalus WA40 strain. The results indicate that the mycocins have potential as biocontrol and biopreservation agents in the poultry industry. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Thu, 21 Aug 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8125 2025-08-21T00:00:00Z https://tede.unioeste.br/handle/tede/8079 Title: Potencial terapêutico dos alcaloides na redução de medidas corporais: uma revisão sistemática Autor: Silva, Robson Ricardo Oliveira da Primeiro orientador: Sanches, Andréia Cristina Conegero Abstract: Obesity is a multifactorial clinical condition characterized by excessive accumulation of body fat and is associated with various chronic diseases. This study a imed togather scientific evidence on the use of alkaloids as a potential therapeutic approach for thetreatment of obesity through a systematic review of randomized clinical trials. The search was conducted in the PubMed, Scopus, Embase, and Web of Science data bases up to February 2025, using descriptors related to obesity and different types of alkaloids. Initially, 300 studies were identified, of which 291 wereselected for title and abstract screening. From these, 56 studies me tthe inclusion criteria and were fully analyzed. The average impact factor of the journals was 4.269, with 78.3% of the studies published in journals with an impact factor equal to or greater than 3. Methodological quality was assessed using the Rob2 Cochrane tool and the Jadad Scale, resulting in an average score of 3.15, indicating moderate to high quality. The results showed that 66.1% of the studies reported significant reductions in body measurements, while 23.2% indicated a tendency to ward reduction. Half of the studies (50%) includedco-interventions suchas diet and physicalactivity. The total sample comprised 3,286 participants, with a mean age of 35.8 years and anaverage treatment duration of approximately 15.8 weeks. Caffeine, aloneor in combination, was the most studied alkaloid, showing predominantly positive results, especially when combined with ephedrine. Berberine demonstrated consistent efficacy acrossall studies in which it was evaluated. Other alkaloids, such as theobromine, piperine, synephrine, and capsaicin, also showed potential for body weight reduction. In contrast, colchicinedid not presentrelevant clinical effects. Weight reduction ranged from 1.65% to 14.4%, depending on the substance or combination used. It is concluded that certain alkaloids may contribute to weight loss, particularly when associated with other interventions. How ever, further studies are necessary to confirm the safety and efficacy of these substances in the treatment of obesity. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Thu, 14 Aug 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8079 2025-08-14T00:00:00Z https://tede.unioeste.br/handle/tede/7974 Title: Avaliação do uso de fibra obtida por eletrofiação contendo óleo de Girassol ozonizado na cicatrização de lesões estéreis Autor: Fabrini, Carla Diel Primeiro orientador: Menolli, Rafael Andrade Abstract: Ozone (O3) is a highly reactive inorganic molecule; that is part of the atmosphere and can also be produced by ozone generators. It is a potent microbicidal agent, having antibacterial, antiviral, antifungal, and antiparasitic properties, which can have its mechanism of action divided into three aspects: immunomodulation, antimicrobial activity, and ability to interact with the oxidant/antioxidant balance. In this regard, ozonated oil has been used topically to treat chronic wounds. The electrospinning technique (or electrostatic spinning) has the potential to produce materials made of nanofibers, and these nanofibers, when assembled, can give rise to blankets and membranes, presenting a large specific surface area, high porosity, and interconnectivity, making them suitable for the nutrient transport, cellular communication, and efficient cellular responses. Wound healing is a multiphase process consisting of three overlapping stages: inflammation, tissue proliferation, and remodeling. Adjuvant therapy is often required because of low healing rates in chronic wounds treated with standard care. Therefore, this work aims to verify the efficiency of ozonized Sunflower oil incorporated into electrospun fibers as a healing method in excisional lesions on the back of BALB/c mice. The electrospun fiber (Ecovio®) showed great promise in reducing the lesion, especially in the initial days (3 days). It was also able to significantly reduce the number of leukocytes in 7 days, showing its anti-inflammatory effect. Sunflower oil, whether ozonated or not, appears to bring greater hydration to the lesion, but further research is needed with different concentrations of ozone in the oil, as there have been controversial results. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Wed, 11 Sep 2024 00:00:00 GMT https://tede.unioeste.br/handle/tede/7974 2024-09-11T00:00:00Z https://tede.unioeste.br/handle/tede/7946 Title: PELLETS DE ÁCIDO TARTÁRICO COMO NÚCLEO MODULADOR DE PH PARA FORMULAÇÕES DE CITRATO DE SILDENAFILA Autor: Bedin, Amanda Moscibroski da Silva Primeiro orientador: Bunhak, Elcio Jose Abstract: Pulmonary arterial hypertension (PAH) is a severe condition characterized by increased pressure in the pulmonary arteries, potentially leading to right heart failure. Sildenafil citrate (SC) is a phosphodiesterase type 5 (PDE5) inhibitor widely used in PAH treatment, but its low solubility and bioavailability limit its clinical efficacy. Therefore, this study aimed to develop a multiparticulate controlled-release formulation of SC, using tartaric acid pellets as the core, solid dispersion, and factorial design to optimize its dissolution and absorption. The methodology involved preparing SC solid dispersions using the Soluplus ® polymer and Tween ® 80 surfactant, followed by incorporating these dispersions into tartaric acid pellets coated with controlled-release polymers. An experimental design (DOE) was applied to assess the influence of variables on drug dissolution. The formulations were characterized using differential scanning calorimetry (DSC), X-ray diffraction (XRD), particle size distribution (PSD), scanning electron microscopy (SEM), and in vitro dissolution analysis with quantification by high-performance liquid chromatography (HPLC). The results demonstrated that solid dispersion significantly improved SC dissolution, and the coated pellets exhibited an adequate controlled-release profile, maintaining drug stability in biological media. Dissolution studies in FaSSIF buffer (pH 6.5) confirmed a gradual SC release, enhancing its absorption in the gastrointestinal tract and improving therapeutic efficacy. Thus, the use of tartaric acid pellets and solid dispersions proved to be a promising strategy to enhance SC’s oral bioavailability and optimize its clinical performance in PAH treatment. This innovative approach may contribute to the development of more effective pharmaceutical formulations, reducing the need for frequent dosing and improving patient adherence to treatment. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Tue, 18 Mar 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/7946 2025-03-18T00:00:00Z