https://tede.unioeste.br/handle/tede/505 Tue, 21 Apr 2026 20:03:19 GMT 2026-04-21T20:03:19Z https://tede.unioeste.br/handle/tede/8366 Title: Análise citogenética de Tetranematichthys wallacei: contribuições à compreensão da evolução cromossômica em Auchenipteridae Autor: CASAROTTO, Cleisson de Cristo Primeiro orientador: Lui, Roberto Laridondo Abstract: Considering the evidence of chromosomal fusion found in Ageneiosus inermis as basal for Ageneiosus, it becomes possible to suppose that this event may have occurred in an ancestor prior to the diversification that defined the groups representing the tribe Agneiosini ((Ageneiosus + Tympanopleura) Tetranematichthys), which can be clarified through the analysis of Ageneiosini species. In addition, in 25 genera currently valid in Auchenipteridae, there are only eight with cytogenetic studies, and of the 21 species of Ageneiosini, only two had been previously studied through cytogenetics analysis. Given this reality, the objective of this research has been to analyze through cytogenetic methods a first sample of the genus Tetranematichthys. For this, 11 individuals (3 females and 8 males) of Tetranematichthys wallacei from the Guamá river basin have been collected and examined. The diploid number found has been 52 chromosomes (32m+18sm+2st), with no differences between the sexes, and with a fundamental number of 104. Blocks of heterochromatin predominantly terminal and some interstitial has been observed. Hybridization with telomeric sequence [TTAGGG]n showed markings only in the terminal regions, without ITS and hybridizations with 18S rDNA showed its presence in pairs 7 metacentric and 17 submetacentric, and pairs 8 and 10 metacentric with 5S rDNA. Only one chromosome of the pair 7 presented 18S rDNA site in only 3 specimens, which constituted a polymorphism. Tetranmatichthys wallacei has some characteristics that call attention to its current phylogenetic position, as it presents a probable reduction in the diploid number in Auchenipteridae. Furthermore, it shares with Tympanopleura atronasus the 18S rDNA allocated on the long arm of a large submetacentric chromosome (first pair), and does not seem to share with Ageneiosus the first metacentric pair with evidence of fusion in A. inermis. The data generated for T. wallacei added to the other two taxa of the tribe previously studied suggest Ageneiosini as the most different clade in the family by now. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Fri, 30 Sep 2022 00:00:00 GMT https://tede.unioeste.br/handle/tede/8366 2022-09-30T00:00:00Z https://tede.unioeste.br/handle/tede/8289 Title: Comportamento de passagem ascendente de Rhinelepis aspera na escada para peixes da UHE Engenheiro Sérgio Motta - CESP, rio Paraná Autor: Borghezan, Hudson Luiz Moret Primeiro orientador: Makrakis, Sergio Abstract: Fish passages were conceived as a tool to mitigate impacts from the dams in rivers, blocking migratory fish routes, such as Rhinelepis aspera, species with high economic value, are found with reduced populations. Based on this the fish passage must be properly monitored for better management, and future planning. Objectives in our work is to evaluate attractiveness of R. aspera in a ladder for fish type tanks with orifices, also identify an efficiency of passage of the fish by the ladder. The study was carried out at the fish ladder at the Engenheiro Sergio Motta, in the Paraná River. There were marked (PIT-tags 32 mm) 200 R. aspera adults. The fish were released equitably at four points downstream of the dam, two left margin and two the right margin. Survival analysis for the evaluation of attractiveness and efficiency of the passage of the species. The entry rate of R. aspera was high, about 70% of the tagged fish entered the ladder. Loose hairs on the four points show a pattern of arrival and arrival rate (Log-Rank: x² = 2.53 and p = 0.46). Only 27% of the R. aspera who entered the ladder were able to effectively reach the exit. In addition, fish that failed to complete a passage through the ladder, remained on average for 36 days on the ladder. These results indicate that a species can easily find the fish ladder, however, the hydraulic characteristics of the ladder may be limiting an ascending passage of the species, thus reducing efficiency. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Wed, 12 Feb 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8289 2025-02-12T00:00:00Z https://tede.unioeste.br/handle/tede/8288 Title: REVISÃO TAXONÔMICA DAS ESPERANÇAS DO GÊNERO ENGONIA (ORTHOPTERA: TETTIGONIIDAE: PHANEROPTERINAE) Autor: Nogueira, Thayla Melissa Gimenez Primeiro orientador: Szinwelski, Neucir Abstract: Tettigoniidae Krauss, 1902 is the most diverse family within Orthoptera, comprising over 8,400 species in 19 subfamilies. Among these, Phaneropterinae Burmeister, 1838 is the richest, with about 3,000 species. Within this subfamily, the tribe Cosmophyllini Brunner von Wattenwyl, 1878 is restricted to the Southern Hemisphere and includes six genera and ten described species, of which only Engonia Brunner von Wattenwyl, 1878 occurs in Brazil. Specimens of Engonia were examined from different localities across Brazil, from the South to the Northeast. Morphological analyses were conducted under a stereomicroscope, and detailed photographs of structures were taken. In addition, Scanning Electron Microscopy (SEM) images of male cercus structures were obtained to refine morphological descriptions. Distribution maps were generated using QGIS software. A total of 143 individuals were analyzed, resulting in the description of 13 new 14 species and the redescription of three previously known ones. Specimens were characterized according to general morphology and tegmen condition (brachypterous, mesopterous, and macropterous) and classified into two groups based on male cercus morphology and the lateral carinae of the pronotum. A dichotomous key for all known species of Engonia was also provided. This comprehensive taxonomic revision expands the known diversity and geographic range of the genus Engonia, contributing to a better understanding of Phaneropterinae diversity and biogeography in the Neotropical region. Key-wor ds Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Wed, 26 Nov 2025 00:00:00 GMT https://tede.unioeste.br/handle/tede/8288 2025-11-26T00:00:00Z https://tede.unioeste.br/handle/tede/8201 Title: Identificação citogenética e molecular de cariomorfos de Hoplias malabaricus (Characiformes, Erythrinidae) na bacia do baixo rio Iguaçu Autor: Perin, Diana Paula Primeiro orientador: Lui, Roberto Laridondo Abstract: Hoplias malabaricus is a complex group in the Erythrinidae family. In the H. malabaricus group there are seven described species. One of these species is H. malabaricus, which is a species complex with seven described karyomorphs. For the Iguaçu watershed, studies related to the distribution of karyomorphs are still scarce and most studies focus on the Upper Iguaçu, while for the middle and lower Iguaçu studies are scarce. Furthermore, the lower Iguaçu suffers anthropic influences, mainly with the introduction of exotic species and the construction of hydroelectric dams due to the high number of waterfalls. Both influences can lead to the extinction of several species. Considering the low number of studies related to karyomorphs of H. malabaricus, and the various issues relevant to this group, the present study aimed to analyze individuals of this species in an integrative way through cytogenetic techniques and barcode DNA. As many sequences as possible from the H. malabaricus group from the Bold System were used in our analyses. Our results showed the presence of three karyotype in sympatry. Two cytotypes of karyomorph A (A1 and A2) and the karyomorph D. Our hypothesis is: 1. Cytotype A1 is the chromosomal form native to the Iguaçu River Basin; 2. Cytotype A2 may represent Hoplias argentinensis specie; 3. Karyomorph D may represent the specie Hoplias misionera; 4. Cytotype A2 and karyomorph D may have been introduced in the Lower Iguaçu River. Our results allowed to increase data regarding the distribution of karyomorphs and species of the H. malabaricus group. In addition to showing that the integration of cytogenetic tools and barcode DNA can be very efficient for the H. malabaricus group. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação Wed, 01 Jun 2022 00:00:00 GMT https://tede.unioeste.br/handle/tede/8201 2022-06-01T00:00:00Z