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Comparação de sistemas rotatórios e manuais na instrumentação dos canais mésio-vestibular e disto-vestibular de segundos molares decíduos superiores: análise por microtomografia computadorizada

Mon, 06 Jul 2026 00:00:00 GMT

Title: Comparação de sistemas rotatórios e manuais na instrumentação dos canais mésio-vestibular e disto-vestibular de segundos molares decíduos superiores: análise por microtomografia computadorizada Autor: Singer, Paulo Henrique Primeiro orientador: Hoshi, Adriano Tomio Abstract: Introduction: Maintaining primary teeth in functional condition until their natural shedding is essential for proper oral development in children. Pulpectomy is a widely recommended treatment option in cases of irreversible pulp damage resulting from extensive carious lesions or dental trauma. However, the complex anatomy of the root canals of primary teeth, especially the upper second molars, poses a significant clinical challenge during the endodontic instrumentation phase. Objectives: To compare the effects of different endodontic instrumentation systems—rotary and manual—on the mesio-buccal and disto-buccal canals of artificial upper deciduous second molars, using computed microtomography analysis. Methodology: Thirty-six artificial teeth were used, divided into three experimental groups (n=12), according to the system employed: Sequence Baby File NiTi (BF), ProDesign Logic 2 (LOGIC), and K-type manual files (MANUAL). After determining the working length, the specimens were instrumented following the manufacturers’ recommendations and subjected to computed microtomography before and after biomechanical preparation. Changes in root canal volume in the cervical, middle, and apical thirds were evaluated, as well as the loss of dentin wall thickness. The data were subjected to statistical analysis, considering a significance level of 5%. Results: the results demonstrated that all systems caused changes in the internal anatomy of the root canals, with differences in the pattern of volumetric increase and dentin wear among the groups. The BF and MANUAL systems exhibited greater volumetric changes in specific regions of the canals, while the LOGIC system demonstrated more homogeneous and conservative behavior. Regardless of the system used, greater wear was observed in the apical third of the mesio-buccal canal, indicating greater structural vulnerability in this region. Conclusion: All systems evaluated are capable of performing biomechanical preparation of root canals in deciduous teeth; however, significant differences exist regarding the preservation of remaining dentin structure, reinforcing the need for careful selection. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação

Mielotoxicidade e hepatotoxicidade da exposição crônica ao herbicida a base de glifosato em camundongos C57BL/6

Mon, 31 Aug 2026 00:00:00 GMT

Title: Mielotoxicidade e hepatotoxicidade da exposição crônica ao herbicida a base de glifosato em camundongos C57BL/6 Autor: Ferreira, Anderson Felipe Primeiro orientador: Bonfleur, Maria Lúcia Abstract: Introduction: The use of glyphosate-based herbicides (GBH) has intensified over the past decades, accompanying the expansion of agricultural production. This scenario has raised concerns regarding the potential health effects of chronic exposure to these compounds. Experimental studies have demonstrated alterations in various biological systems following exposure to glyphosate and commercial formulations containing this herbicide, including hepatic and hematological effects. However, the effects of chronic exposure on the bone marrow and the mechanisms involved in hematotoxicity remain incompletely understood. Objective: To evaluate the effects of chronic exposure to 5 and 50 mg/kg doses of a commercial GBH formulation on hematological parameters, bone marrow, and liver of male C57BL/6 mice. Methods: Male C57BL/6 mice, approximately 21 days old and with a mean body weight of 20 g, were distributed into three experimental groups: control (CTL), 5 mg/kg GBH (GBH-5), and 50 mg/kg GBH (GBH-50). The mice received the commercial formulation Xeque Mate HT® diluted in drinking water ad libitum for 20 weeks. At the end of the experimental period, following euthanasia, body profile assessment, hepatic histopathological analysis, complete blood count and reticulocyte count, cytological and histological evaluation of the bone marrow, and serum thrombopoietin (TPO) measurement were performed. Results: GBH exposure increased water and food consumption, without significantly affecting final body weight gain. Hepatic histopathological evaluation revealed hepatocellular hypertrophy, inflammatory infiltrate, and increased connective tissue. Regarding hematological evaluation, no relevant alterations in erythropoiesis were observed; however, alterations in the leukocyte profile were identified, with more pronounced leukocytosis, neutrophilia, and monocytosis in both GBH-exposed groups. In addition, platelet counts were reduced in the GBH-5 and GBH-50 groups compared with the CTL group, by 26.39% and 46.45%, respectively. Bone marrow evaluation revealed a significant increase in megakaryocyte numbers in the GBH-50 group compared with the CTL and GBH-5 groups, along with dysplastic alterations consistent with impaired megakaryopoiesis. Serum TPO concentrations remained unchanged among the experimental groups. Conclusions: Chronic exposure to 5 and 50 mg/kg doses of a commercial GBH formulation promoted hepatic and hematological alterations, as well as changes in the bone marrow of C57BL/6 mice. The main findings were thrombocytopenia associated with dysmegakaryocytopoiesis, with no change in serum TPO concentrations. These findings suggest ineffective thrombopoiesis as one of the mechanisms involved in herbicide-induced hematotoxicity. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação

Concordância na avaliação da mudança de cor entre o guia de cores VITA Classical e o espectrofotômetro VITA Easyshade após o clareamento dental em consultório: uma revisão sistemática e meta análise

Thu, 02 Jul 2026 00:00:00 GMT

Title: Concordância na avaliação da mudança de cor entre o guia de cores VITA Classical e o espectrofotômetro VITA Easyshade após o clareamento dental em consultório: uma revisão sistemática e meta análise Autor: Struckel, Bárbara Brusco Primeiro orientador: Camilotti, Veridiana Abstract: Introduction: Accurate assessment of tooth color change is essential for evaluating the effectiveness of in-office bleaching procedures. Although visual and instrumental methods are widely used, the agreement between the VITA Classical shade guide and the VITA Easyshade spectrophotometer remains unclear. Objective: To systematically review the literature and perform a meta-analysis to evaluate agreement and systematic differences in color change assessment between the VITA Classical shade guide and the VITA Easyshade spectrophotometer following in-office tooth bleaching with 35% hydrogen peroxide. Methodology: The protocol was registered in PROSPERO (CRD42024476136). Searches were conducted in MEDLINE, Cochrane Library, Embase, LILACS/BBO, Scopus, Web of Science, grey literature and clinical trial registries. Randomized clinical trials (RCTs) evaluating both methods in the same participants were included. Risk of bias was assessed using the Cochrane (RoB 2.0). Agreement was evaluated using Bland Risk of Bias 2.0 Altman analysis. Differences between methods were analyzed using standardized mean differ ences (SMD) with a random-effects model. Subgroup, meta-regression and equivalence testing (TOST; ±0.30 SMD) were performed. Results: Twenty-two RCTs were included. The VITA Easyshade spectrophotometer detected significantly greater color change than the VITA Classical shade guide (SMD = 1.05; 95% confidence interval [CI]: 0.79–1.30; p < 0.00001). Bland–Altman analysis showed systematic positive bias (+3.8 ΔE*) and wide limits of agreement, indicating low agreement. No significant subgroup or meta-regression effects were observed. Sensitivity analyses confirmed robustness. Equivalence testing confirmed non equivalence. Conclusions: The VITA Classical shade guide and the VITA Easyshade spectrophotometer are not equivalent and should not be used interchangeably for assessing color change after in-office bleaching. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação

Solanaceae no Parque Nacional do Iguaçu, Paraná, Brasil

Thu, 29 May 2025 00:00:00 GMT

Title: Solanaceae no Parque Nacional do Iguaçu, Paraná, Brasil Autor: Munaro, Izabele Oliveira Primeiro orientador: Temponi, Lívia Godinho Abstract: Solanaceae comprises 98 genera and 2300 species worldwide, exhibiting great morphological and chemical diversity, with South America as its primary center of diversification. In Brazil, there are 515 species and 36 genera, occurring in various plant formations, with greatest richness in Atlantic Forest formations. In Paraná, several records exist for its representatives, but detailed surveys of its diversity, especially within Conservation Units, remain scarce. Iguaçu National Park (ParNa Iguaçu) harbors the largest continuous remnant of the Atlantic Forest in the interior and is essential for biodiversity conservation. This study aimed to investigate the richness of Solanaceae in ParNa Iguaçu, contributing to the expansion of taxonomic and floristic knowledge in the region. Botanical material was collected along trails and park edges, in areas of Semideciduous Seasonal Forest (FES) and Mixed Ombrophilous Forest (FOM), using the walking method, followed by herborization and sample inclusion in the UNOP e EVB Herbarium. Additionally, an extensive survey of herbarium material was conducted through the SpeciesLink, JABOT e REFLORA platforms, along with in-person access to the collections of EVB, HCF, MBM and UNOP The determination, description of species, and construction of the dichotomous identification key were based on the analyzed samples and specialized literature. A total of 37 species were recorded, distributed across 12 genera, with Solanum (21 spp.), Cestrum (3 spp.), and Brunfelsia (3 spp.) being the most representative. The species found in ParNa Iguaçu are all native to Brazil, except Brugmansia suaveolens, which is naturalized. Some species, such as Athenaea fasciculata and C. mariquitense, were found only in FES areas, while S. paniculatum and S. cylindricum are restricted to FOM. Only Brunfelsia australis and B. cuneifolia are considered endemic to the country, while Petunia guarapuavensis, S. corymbiflorum, and S. fusiforme occur only in the southern region. This study revealed 17 new occurrences for the study area, highlighting Athenaea fasciculata, Lycium glomeratum, S. chacoense, S. delicatulum, S. fusiforme, S. paniculatum, S. pseudocapsicum, S. robustum, S. sanctae-catharinae, and Vassobia breviflora which showed records limited to one or two observations. The obtained data reinforce the importance of ParNa Iguaçu as a repository of regional biodiversity, highlighting the relevance of continuing studies that deepen the knowledge of the local flora. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação