https://tede.unioeste.br/handle/tede/603 2026-07-13T22:09:45Z https://tede.unioeste.br/handle/tede/8503 Title: Prospecção de bactérias produtoras de biossurfactantes em áreas de transição (portos) Autor: Batista, Paula Frassinetti da Silva Primeiro orientador: Makrakis, Sergio Abstract: Biosurfactants, amphiphilic compounds produced by microorganisms, have attracted increasing interest due to their biodegradability, low toxicity, and effectiveness under diverse environmental conditions, making them promising alternatives to synthetic surfactants in remediation processes and industrial applications. This dissertation is structured into two complementary chapters. The first chapter presents a systematic review on the prospecting of biosurfactants in transitional ecosystems, such as mangroves, port areas, and coastal zones, with emphasis on their applications in bioremediation. Sixteen articles published between 2015 and 2025 were analyzed, selected from international databases (Scopus, Web of Science, and PubMed). The studies indicate a predominance of biosurfactants belonging to the glycolipid and lipopeptide classes, produced mainly by bacteria of the genera Pseudomonas, Bacillus, and Rhodococcus, as well as yeasts such as Candida and Starmerella. A growing trend toward the use of agro-industrial residues as substrates was also observed, reinforcing the alignment of these technologies with sustainability principles and the circular economy. The reported applications demonstrate high efficiency of biosurfactants in the solubilization and mobilization of pollutants, including hydrocarbons and heavy metals, highlighting their strategic role in the bioremediation of impacted transitional environments. In the second chapter, an experimental study was conducted to prospect biosurfactant-producing bacteria in port areas of the municipality of Piúma, Espírito Santo, Brazil, characterized by contamination from diesel oil. Sediment samples were collected from points of high vessel traffic, resulting in the isolation of seven bacterial strains. These isolates were cultivated in minimal salt medium (MSM), using diesel oil as the sole carbon source. The surfactant activity of the produced metabolites was evaluated using drop-collapse and oil-spreading tests, as well as the emulsification index (E24), employing diesel oil and gasoline as oily phases. The biosurfactants exhibited low emulsifying activity against diesel oil (E24 < 10%); however, they demonstrated moderate efficiency in the emulsification of gasoline, with indices reaching approximately 35% for some isolates, indicating greater affinity for light hydrocarbons. Taken together, the findings from the systematic review and the experimental study reinforce the potential of biosurfactants as valuable tools for the remediation of contaminated environments and highlight the importance of transitional ecosystems— such as mangroves and port areas—as reservoirs of microorganisms with biotechnological relevance. The integration of these approaches contributes to advancing knowledge on the microbial diversity involved in biosurfactant production, their applications, and the remaining challenges related to large-scale production and the economic feasibility of these bioproducts in environmental and industrial contexts. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação 2025-12-01T00:00:00Z https://tede.unioeste.br/handle/tede/8463 Title: Liofilização de produtos aquáticos: tendências científicas, desafios atuais e influência da geometria de corte na qualidade de filés de tilápia-do-Nilo (Oreochromis niloticus) Autor: Souza, Pauliana Leão de Primeiro orientador: Signor, Altevir Abstract: In this review, we synthesize the literature on the freeze-drying of aquatic products to analyze the current state of research on this topic worldwide. In total, 345 publications met the inclusion criteria. It was observed that, from 1958 onwards, the volume of published studies grew considerably, distributed across 181 journals in the areas of Analytical Chemistry, Biochemistry, and Food Science and Engineering. The journals Food Chemistry (6.96%), International Journal of Biological Macromolecules (2.90%), Journal of Food Science (2.90%), and Journal of the Science of Food and Agriculture (2.90%) concentrated the largest number of publications. The study of freeze-drying in aquatic products has sparked the interest of researchers in 51 countries. However, scientific output has been concentrated in China (21.74%) and the United States (8.70%). In contrast, publications originating from Oceania, Africa, and Central America have remained scarce. Furthermore, although the research covered 12 different types of raw materials, fish (64.68%) was the main target of the investigations. Among the fish species, Nile tilapia (Oreochromis niloticus) was the one with the greatest interest for the application of the freeze-drying method (7.20%) in the studies. There was a predominance of studies involving muscle tissues and their derivatives (41.13%) and protein biomolecules (23.38%). The focus of most of the research was related to the areas of Food Engineering (48.93%) and Analytical Chemistry (17.91%). Predominance observed in most of the countries analyzed. However, although the scope of the studies has changed over the years, most publications have remained in already established areas. Furthermore, research is needed to reduce energy costs and improve equipment, aiming to expand access to freeze-drying in regions where its use is still limited. In addition, it is essential to delve deeper into underexplored fields such as Animal Health and Genetics, as well as investigations into the freeze-drying of aquatic by-products, with the goal of adding value and ensuring the sustainability of the process. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Tese 2026-03-09T00:00:00Z https://tede.unioeste.br/handle/tede/8449 Title: Socioeconomia e qualidade de vida dos pescadores profissionais do entorno do Parque Nacional de Ilha Grande MS/PR, Brasil Autor: Yunes, Rosinara Virginia Ferreira Primeiro orientador: Kashiwaqui, Elaine Antoniassi Luiz Abstract: This research investigates the socioeconomic conditions and quality of life of professional fishers operating in the surroundings of Ilha Grande National Park, located in the Upper Paraná River Basin, a region marked by profound environmental transformations resulting from river damming and the establishment of protected areas. Developed in a multipaper format, the dissertation articulates two complementary empirical studies based on interviews with 59 professional fishers, combining descriptive analysis, content analysis, and multidimensional perceptual assessment. The first study characterizes the socioeconomic and productive profile of these fishers, highlighting the aging of the category, low educational attainment, economic dependence on fishing, declining income, and changes in fish composition associated with historical damming processes along the Paraná River. The second study deepens this analysis by evaluating fishers’ quality of life across economic, social, and environmental dimensions, comparing the fishers’ own perceptions with assessments of institutional governance. The findings reveal significant divergences between these rationalities, indicating that quality of life cannot be understood through isolated or exclusively institutional indicators, but rather as a multidimensional construct shaped by environmental, economic, and social factors, as well as by traditional fishing knowledge. By shedding light on tensions between environmental conservation, public policies, and traditional ways of life, this research contributes to the debate on professional fishing in protected areas and to the formulation of management strategies that are more responsive to the social realities of fishing communities. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Tese 2026-03-09T00:00:00Z https://tede.unioeste.br/handle/tede/8393 Title: Revisão sistemática a respeito de off-flavors causados por Geosmina e 2-Metilisoborneol (MIB) em produtos aquáticos Autor: Santos, Osvaldo Gois Primeiro orientador: Ballester, Eduardo Luis Cupertino Abstract: This systematic review addresses the issue of sensory quality of freshwater fish farmed by aquaculture. Off-flavors are undesirable flavors and odors in farmed fish that affect the quality and acceptance of the product by the consumer, causing serious financial losses to the production chain. The PRISMA method was used to select articles related to the study theme, where 7,576 documents were analyzed, opting for recent articles, published in the last six years, obtained from the Scopus and Web of Science databases. After applying the exclusion terms (off-flavor, aquaculture, fish, articles, periods, GSM and MIB), forty-four relevant studies were obtained, in which we sought to identify the objectives of the study, the species studied, the farming system, results achieved, gaps and future studies of each investigated article, briefly reporting their objectives and results. Most studies emphasize the influence of water quality, climate issues, management, feed quality, especially the presence of cyanobacteria, algal blooms, fungi, and metabolic compounds such as GSM, MIB, among others, which contribute to the formation of unpleasant flavors and odors. The results highlight the need to improve management practices and control of altered flavors and odors in aquaculture, in addition to suggesting the need for further research to develop more effective and accessible methods. The importance of continuous monitoring of water quality is emphasized to prevent the formation of undesirable flavors. In summary, the review provides a comprehensive overview of undesirable flavors and odors in freshwater fish, identifying gaps in knowledge and proposing directions for future research, essential for the sustainability and quality of aquatic production. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação 2025-02-14T00:00:00Z