https://tede.unioeste.br/handle/tede/514 2026-08-15T11:02:07Z https://tede.unioeste.br/handle/tede/8611 Title: Desenvolvimento de um simbiótico com base de biomassa de banana verde Autor: Picoli, Leticia Saifert Primeiro orientador: Corrêa, Juliana Moço Abstract: Functional food with symbiotic characteristics enrich the diet with prebiotic substances. It means that non-digested substances that upon reaching the colon, are selectively metabolized by a limited number of beneficial bacteria, which alter the colon by generating a healthy bacterial flora and of living microorganisms (probiotics) capable of surviving the passage through the gastrointestinal tract. Banana (Musa sp), when still green, is a fruit rich in resistant starch (SR), a starch fraction that does not provide free glucose to the body and resists to the enzymatic digestion in the small intestine. Resistant starch when fermented in large intestine, mainly by the bifidobacteria, produces gases and fatty acids of short chain, presenting behavior similar to the dietary fiber, found in the prebiotics. Aiming the development of new alternatives of symbiotic products, this paper has developed a symbiotic with green banana basis. In order to explore green banana biomass potential as a possible prebiotic, were analysed the bromatological characteristics such as pH, acidity, moisture and ashes, phytochemical identification through the visual identification test of phenolic compounds, Shinoda test, quantitative test of total phenols the Folin Ciocalteau, extraction of carotenoids by the combination of acetone/petroleum ether and acetone / hexane solvents, as well as quantification by spectrophotometer with reading at 450 nm. The antioxidant activity was evaluated by the 2,2-diphenyl-2 picrylhydrazyl (DPPH) test and the ABTS free radical capture capacity. Five varieties of green banana were used for the production of biomass. The results have presented that the biomass produced with the apple variety has the highest antioxidant percentage with 91.96% in DPPH and 87.46% in ABTS, physico-chemical and phytochemical results show that even after the cooking process, the presence of vitamins, phenolic compounds and carotenoids remain present. The study also resulted in a symbiotic commercial product in the form of a green banana biomass based gelatinous gum where the antioxidant capacity of 68.19% for Bifidobacterium enriched gum and 22.28% for gum enriched with Lactobacillus. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação 2019-05-03T00:00:00Z https://tede.unioeste.br/handle/tede/8610 Title: Atividade antioxidante de sementes de Eugenia involucrata dc. e potencial aplicação cosmética Autor: Cherubim, Débora Jackeline de Lima Primeiro orientador: Lucca, Rosemeire Aparecida da Silva de Abstract: In the XXI century, the valorization of Brazilian biodiversity and the commitment to sustainable development has stimulated the preference for products of natural sources as an alternative to the use of synthetic products. In this scenario, the plant extratcs application in cosmetic formulations is a promising trends in the market, which demands new sustainable and economical sources for functional raw materials. The Eugenia involucrate DC. is a native plant to southern Brasil region, belonging to the Myrtaceae family. This genus stands out because it has a large amount of phenolic compounds in its composition, with proven antimicrobial, anti inflammatory and antioxidant capacity. The objective of this study was to evaluate the antioxidant activity and the phenolic composition of aqueous and hydroalcoholic extracts obtained from the Eugenia involucrata seeds, as well as to apply the obtained extract in a cosmetic formulation. The study included Folin-Ciocalteau reducing capacity, DPPH and ABTS to determine the antioxidant activity and antimicrobial activity by the disc-diffusion method. Toxicity was determined by hemolytic assay, hen’s egg test - chorioallantoic membrane (HET-CAM) and Artemia salina assay. The stability studies were evaluated according to the Stability Guide for Cosmetic Products. In general, the hydroalcoholic extract presented the best results, with a highest content of phenols (116,3 ± 0,6 mg EAG/g), expressive antioxidant capacity (IC50 around 0.06 mg/ml) and significant antimicrobial activity. The results demonstrated a not significant toxicity in human erythrocytes, CAM and Artemia salina. In relation to the cosmetic application, the formulations containing the extracts presented superior activity to those containing the synthetic antioxidant. It was concluded that the hydroalcoholic extract presents as a promising antioxidant, since it demonstrated absence of toxicity, low irritability potential and relevant antioxidant effect when applied to the cosmetic formulation, besides maintenance of organoleptic and physicochemical characteristics practically unchanged during stability studies. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação 2019-03-26T00:00:00Z https://tede.unioeste.br/handle/tede/8607 Title: A influência dos efeitos anti-hipertensivos da tintura da planta Aloysia polystachya (Griseb) Moldenke (Verbenaceae) na pressão arterial de pacientes hipertensos Autor: Santos, Priscila Helena Pereira dos Primeiro orientador: Fariña, Luciana Oliveira de Abstract: Arterial Hypertension (AH) is a multifactorial and modifiable clinical condition that affects a large number of the world's population. Aloysia polystachya (Griseb) Moldenke (Verbenaceae), native plant from Argentina, commonly known as Burrito and it is used due to its digestive, calming, and antihypertensive effects. Previous studies have demonstrated positive effects in antihypertensive treatment, but there is no clinical evidence of its use in humans, even though it has been already used in popular medicine. Thus, this study aimed at evaluating the effects of A. polystachya tincture on blood pressure and anxiety profiles of participants from the Morumbi Educational Institute (IEM), as well as at promoting community pharmaceutical assistance actions. This project was approved by the Research Ethics Committee of the Western Paraná State University - UNIOESTE (CAAE: 85465324.5.0000.010). Two interventions were carried out. The first intervention consisted of a randomized, double-blind, placebo-controlled clinical trial with 18 hypertensive participants, followed for 30 days. Blood pressure was measured weekly following a standardized protocol. An average reduction of 13.70 mmHg in systolic blood pressure and 10.94 mmHg in diastolic blood pressure was observed, a magnitude considered clinically relevant and consistent with data from the literature. The second intervention included thirteen participants who performed self monitoring of blood pressure and used the tincture for fifteen days. Only five participants submitted their complete data due to low adherence. So, it was observed an average reduction of 9.55 mmHg in systolic blood pressure, with significant interindividual variability. Anxiety levels were assessed using the Beck Anxiety Inventory (BAI), applied before and after fifteen days of tincture use. Four participants showed a slight decrease in scores, which suggested an anxiolytic effect limited by the short intervention period. These answers are in accordance with previous studies that indicate more consistent effects after prolonged use period. Alongside the clinical study, pharmaceutical care actions were developed, including educational lectures, blood pressure measurement, anthropometric surveys, and the creation of a community health profile. It is concluded, therefore, that A. polystachya tincture presents potential antihypertensive and anxiolytic effects; however, studies with larger samples, longer durations, and better adherence control are necessary to validate the findings and expand its clinical applicability. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação 2026-02-24T00:00:00Z https://tede.unioeste.br/handle/tede/8588 Title: Fórmulas infantis prebióticas: caracterização físico-química, microbiológica e verificação do efeito prebiótico Autor: Soares, Mykelli De Andrade Santos Primeiro orientador: Bernardi, Daniela Miotto Abstract: Infant formulas (IF) constitute an important nutritional alternative for infants when breastfeeding is not possible, making it essential to ensure their physicochemical, microbiological, and functional quality. This study aimed to characterize prebiotic infant formulas intended for infants aged 0 to 6 months regarding their physicochemical composition, microbiological quality, and their ability to stimulate probiotic growth in vitro. Three commercial brands of IF supplemented with fructooligosaccharides (FOS) and galactooligosaccharides (GOS), acquired from retail stores in Cascavel, Paraná, Brazil, were evaluated. Physicochemical and microbiological analyses were performed on powdered and reconstituted formulas immediately after the package was opened and after storage periods at room temperature. The growth of Lactobacillus acidophilus in media supplemented with these formulas was also evaluated. The results demonstrated that the IF presented centesimal composition within the limits established by Brazilian legislation, although significant differences among brands and batches were observed in some parameters. Microbiological analyses showed the absence of pathogenic microorganisms of sanitary relevance, indicating satisfactory microbiological safety conditions throughout the evaluated period. A slight increase in mesophilic aerobic counts was observed during storage of the reconstituted formulas; however, the values remained within acceptable standards. Formulas supplemented with prebiotics promoted the growth of Lactobacillus acidophilus, demonstrating a potential in vitro prebiotic effect. It was concluded that the evaluated prebiotic infant formulas presented satisfactory physicochemical and microbiological quality, in addition to functional potential related to the stimulation of beneficial bacterial growth, reinforcing their importance as a nutritional alternative for infants. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação 2026-07-28T00:00:00Z