https://tede.unioeste.br/handle/tede/515 2026-08-07T06:00:32Z 2026-08-07T06:00:32Z Soares, Mykelli De Andrade Santos https://tede.unioeste.br/handle/tede/8588 2026-08-05T19:15:31Z 2026-07-28T00:00:00Z

Title: Fórmulas infantis prebióticas: caracterização físico-química, microbiológica e verificação do efeito prebiótico Autor: Soares, Mykelli De Andrade Santos Primeiro orientador: Bernardi, Daniela Miotto Abstract: Infant formulas (IF) constitute an important nutritional alternative for infants when breastfeeding is not possible, making it essential to ensure their physicochemical, microbiological, and functional quality. This study aimed to characterize prebiotic infant formulas intended for infants aged 0 to 6 months regarding their physicochemical composition, microbiological quality, and their ability to stimulate probiotic growth in vitro. Three commercial brands of IF supplemented with fructooligosaccharides (FOS) and galactooligosaccharides (GOS), acquired from retail stores in Cascavel, Paraná, Brazil, were evaluated. Physicochemical and microbiological analyses were performed on powdered and reconstituted formulas immediately after the package was opened and after storage periods at room temperature. The growth of Lactobacillus acidophilus in media supplemented with these formulas was also evaluated. The results demonstrated that the IF presented centesimal composition within the limits established by Brazilian legislation, although significant differences among brands and batches were observed in some parameters. Microbiological analyses showed the absence of pathogenic microorganisms of sanitary relevance, indicating satisfactory microbiological safety conditions throughout the evaluated period. A slight increase in mesophilic aerobic counts was observed during storage of the reconstituted formulas; however, the values remained within acceptable standards. Formulas supplemented with prebiotics promoted the growth of Lactobacillus acidophilus, demonstrating a potential in vitro prebiotic effect. It was concluded that the evaluated prebiotic infant formulas presented satisfactory physicochemical and microbiological quality, in addition to functional potential related to the stimulation of beneficial bacterial growth, reinforcing their importance as a nutritional alternative for infants. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação

2026-07-28T00:00:00Z Webler, Emanuelle Menegazzo https://tede.unioeste.br/handle/tede/8580 2026-08-03T18:54:54Z 2015-12-09T00:00:00Z

Title: Desenvolvimento de complexos de inclusão bosentana-ciclodextrina e avaliação das características biofarmacêuticas. Autor: Webler, Emanuelle Menegazzo Primeiro orientador: Rosa, Mauricio Ferreira da Abstract: Pulmonary hypertension is a condition that affects the respiratory system, causing fatigue and exercise intolerance. In addition to being an incurable disease, its evolution is fast, and there are few effective options for treatment. The need for drugs to treat this disease and the growing interest of the medical class the knowledge of this disease prompted the study in this area, in order to search for therapeutic enhancement of currently available drugs for the treatment of pulmonary arterial hypertension. Because the bosentan a low solubility and bioavailability of drug, is interesting that pharmacotechnical methods are developed to provide improved absorption of this drug and potentiation of pharmacological activity of the therapeutic dose approved for the treatment of pulmonary hypertension. One of the available techniques for improving solubility and bioavailability of drugs is complexation with cyclodextrins used successfully in low aqueous solubility drugs. Thus, the intention of developing bosentan inclusion complexes with cyclodextrins is to promote better therapeutic use, increasing the effectiveness of the pharmacological treatment of patients with pulmonary hypertension. This research proposes the formation of bosentan inclusion complexes and cyclodextrins to increase drug solubility and improve the bioavailability of the drug, providing a differentiated ability to potentiate the treatment of pulmonary hypertension, can promote drug absorption, by optimizing the therapeutic effect and improving the quality of life of patients. The inclusion complex was prepared by several techniques, in solution, by physical mixing and spray-drier, evaluating the physico-chemical changes and characterization of the complex through the kinetic techniques, the stoichiometry of complexation, the solubility isotherms, spectrophotometry UV-VIS, solubility, DSC, TGA, infrared spectroscopy, electron microscopy in bright field and polarized light, apparent dissolution and dissolution profile. The results showed the formation of the inclusion complex due to changes in the characteristic peaks of the raw materials and increasing solubility, taking into consideration the conditions evaluated. The inclusion complex showed higher water solubility that the isolated bosentan, reinforcing the possibility that a formulation with the inclusion complex may have greater bioavailability in the organism. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação

2015-12-09T00:00:00Z Spohr, Franciele Carline https://tede.unioeste.br/handle/tede/8577 2026-07-31T14:45:48Z 2026-05-22T00:00:00Z

Title: Suplementos Alimentares: aspectos legais, sanitários e os riscos à saúde pública. Autor: Spohr, Franciele Carline Primeiro orientador: Fariña, Luciana Oliveira de Abstract: This dissertation aimed to analyze the legislative compliance of the commercialization of dietary supplements based on medicinal plants requiring medical prescription, according to the Herbal Formulary of the Brazilian Pharmacopoeia, marketed in grain stores and natural product shops in the municipality of Medianeira, Western Paraná, Brazil. The study was based on the hypothesis that products containing substances with pharmacological potential were being marketed as dietary supplements, often in disagreement with current health regulations, potentially posing risks to public health, especially in situations requiring medical prescription and professional monitoring. Despite the increasing consumption of dietary supplements and herbal products in Brazil, their integration into clinical practice and the regulatory context still showed limitations and challenges related to safety, inspection, and rational use. The research adopted a qualitative and quantitative approach through literature review and a descriptive observational field study. Aspects related to labeling, regulatory classification, therapeutic claims, presence of medicinal plant mixtures, and commercialization practices of the identified products were evaluated. The level of knowledge of the establishment managers regarding the regulations applicable to dietary supplements and medicinal plants was also analyzed. The results revealed the commercialization of products containing medicinal plants requiring medical prescription presented as dietary supplements, as well as the presence of nonconformities related to labeling, sanitary designation, and regulatory classification. The offer of substances requiring medical prescription without proper professional guidance was also identified. In addition, therapeutic claims potentially incompatible with the regulatory category of dietary supplements were observed. These findings demonstrated weaknesses in compliance with current health legislation and gaps in technical knowledge regarding the use of medicinal plants and dietary supplements. The study also highlighted the importance of strengthening regulation, intensifying health surveillance actions, and promoting appropriate guidance for both establishment owners and consumers, aiming to encourage the rational use and responsible commercialization of these products, which are often perceived as “natural” and therefore risk-free, although this perception does not always reflect reality. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação

2026-05-22T00:00:00Z Zismann, Samuel Angelo https://tede.unioeste.br/handle/tede/8576 2026-07-31T13:54:33Z 2021-12-07T00:00:00Z

Title: Uma breve revisão: aspectos metodológicos e estruturais de peptídeos antimicrobianos (PAMs) de fungos filamentosos e suas aplicações biotecnológicas. Autor: Zismann, Samuel Angelo Primeiro orientador: Silva, José Luis da Conceição Abstract: Antimicrobial peptides (AMPs) are small proteins that have activity against invaders or that compete for the same survival environment. AMPs are usually composed of 10 to 50 amino acids, with an overall positive charge, ranging from +2 to +11, most often in the α-helix or ẞ-sheet conformation. Among similar producers are filamentous fungi, the phylum Ascomycota possesses the majority of producers. The production of AMPs (called AFPs) by the genus Aspergillus and Penicillium stands out. Studies have been undertaken with the purpose of growing the levels of AFPs production and its expression, in a way that is possible to evaluate the molecules structurally and to understand the factors that lead to biological activity. This work aimed to present, through literature review, the biochemical characteristics, the techniques of production, and purification of the main AFP, produced by filamentous fungus described in the literature, with emphasis on Aspergillus and Penicillium genera, as well as the application of these proteins. A search for articles and information was carried out in the PubMed database, Portal de Periódicos da Capes and Proteins of the National Center of Biotechnology Information (NCBI), retrieving articles between the period of 1965 and 2021. Currently, there is a production of AFP by culturing the organism in a specific culture media that induces the fungus to the desired product, and by means of an expression system based on the P. chrysogenum. The purification of the peptides occurs mainly through chromatographic techniques, usually ion exchange due to the cationic nature of the peptides. Once produced and purified, AFPs have the possibility of being explored in terms of their structure and application. The chitin-binding sequence (in peptides of Aspergillus genus), the γ-core motif, the disulfide bonds and the positive charge are highlighted as key structures of these molecules, allowing the use of these proteins in the fight against fungal infection in agriculture and in the human body. Since the difficulty in isolating AFPs due to the lack of expression of these molecules or the expression at low levels seems to have been overcome by genetic engineering, possibly the transition to in vivo and large-scale studies is the biggest challenge today, since the studies for the application of AFPs remain at the laboratory level (in vitro), wich requires not only studies that elucidate the structure and function of these proteins, but also efforts that allow the arrival of AFPs in the industry. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Dissertação

2021-12-07T00:00:00Z