https://tede.unioeste.br/handle/tede/507 2026-09-24T11:01:29Z 2026-09-24T11:01:29Z Bandeira, Debora Marina https://tede.unioeste.br/handle/tede/8668 2026-09-23T19:43:32Z 2025-03-20T00:00:00Z

Title: Avaliação funcional e potencial biotecnológico da água residuária de agroindústrias de mandioca: do isolamento à análise metagenômica Autor: Bandeira, Debora Marina Primeiro orientador: Gomes, Simone Damasceno Abstract: Cassava is recognized as an agricultural crop of great importance in Brazil, which generates by-products with a high organic load. The toxicity of the wastewater associated with cassava processing is limited to the excess of organic compounds and sugars and the presence of cyanoglycosides, which pose environmental risks if not adequately treated. However, this wastewater has significant potential as a source of nutrients and bioactive compounds for sustainable biotechnological applications, mitigating adverse environmental impacts. This study evaluated wastewater samples from two cassava processing agro-industries: a starch processor (ARFe) and a flour processor (ARFa). The aim was to investigate this waste's microbial composition and biotechnological potential using microbiological isolation methods, metagenomics, and functional tests. In article 1, bacterial isolates were obtained using the serial dilution and isolation method, followed by morphological and Gram stain characterization and physiological characterization regarding biological nitrogen fixation, phosphate and potassium solubilization, and siderophore synthesis. A total of 182 bacterial isolates were obtained, which demonstrated functional capacities relevant to agriculture, including a high rate of nitrogen fixation, phosphate and potassium solubilization, and siderophore production, especially in the ARFa isolates, which indicates potential for the development of biofertilizers. Article 2 carried out a metagenomic analysis of wastewater, which revealed a predominance of the Proteobacteria, Bacteroidota, and Firmicutes phyla, with a greater abundance of the Gemmatimonas, Nannocystis, and Bacillus genera. Despite the chemical variations between the samples, the taxonomic and functional profiles remained stable from 2022 to 2024. The significant correlation between functional diversity and substrate availability reinforces the direct impact of the physicochemical factors of each wastewater on the microbial structure and metabolic behavior of the microorganisms. In the third article, fermentation experiments were carried out using wastewater as a substrate for the bacterium Priestia aryabhattai (CCMMIAB UNIO423) to produce indole-3-acetic acid (IAA) under different supplementation conditions (S1 and S2). Both samples sustained exponential microbial growth; ARFa showed faster initial growth, followed by a decline associated with nutrient depletion, while ARFe showed more stable and prolonged growth over time. S2 supplementation (casein, urea, sucrose, and potassium phosphate) resulted in greater cell viability compared to S1 (glycerol, ammonium nitrate, and yeast extract), with a high production of indole-3-acetic acid (IAA), especially in ARFa. The results indicate the biotechnological potential of cassava wastewater for applications in bioremediation and the production of biofertilizers. The study reinforces the feasibility of using these effluents as a substrate for the growth of plant growth-promoting microorganisms, which contributes to reducing environmental impact and promotes sustainability in agricultural production chains. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Tese

2025-03-20T00:00:00Z Oliveira, Daiane de Deus https://tede.unioeste.br/handle/tede/8667 2026-09-23T20:11:59Z 2025-09-04T00:00:00Z

Title: Fontes de Silício e Corretivos na Agricultura: efeitos sobre os atributos químicos e físicos do solo e na produtividade do trigo, milho e cana-de-açúcar Autor: Oliveira, Daiane de Deus Primeiro orientador: Maggi, Marcio Furlan Abstract: The growing global concern over increasing food demand has led to the expansion of new agricultural areas. However, environmental issues—such as the reuse and recycling of waste discarded by industries—have prompted reflection on how to boost agricultural productivity while ensuring both economic gain and environmental sustainability. In this context, new technologies have been refined and implemented in the agricultural sector with the goal of reducing production costs and increasing crop yields. One notable example is precision agriculture, which uses tools such as electrical conductivity measurements combined with chemical and physical soil analyses. These techniques contribute to sustainable and balanced management practices, preventing drift and leaching of many agricultural inputs, as well as ensuring the correct application of fertilizers. For this reason, the first chapter of the thesis evaluated whether there was any interference in the chemical and physical properties of the soil in relation to electrical conductivity and the productivity of wheat and corn crops after the application of limestone and silicate. This phase of the study was carried out on a commercial agricultural property located in the municipality of Céu Azul, in the state of Paraná, Brazil. After soil collection and chemical analysis, application rates were established at 0, 300, and 800 kg·ha⁻¹ for the control, silicate, and limestone treatments, respectively. To measure soil electrical conductivity, an EM38-MK2 conductivity meter was used. Data cleaning, interpolation, and correlation analyses were conducted using the AgDataBox platform. Results showed that only the nutrient calcium presented a significant difference between the silicate and control treatments, based on the chemical sampling conducted in 2022. Regarding the correlation matrix, autocorrelation among chemical elements was observed; however, there was no correlation between electrical conductivity and magnesium in the 2024 crop season. Additionally, autocorrelation was found between soil compaction resistance and different depths, soil texture, and yield levels. A significant difference was also observed in soil compaction resistance between the control and silicate treatments. It was concluded that both positive and negative correlations, as well as autocorrelations, existed between the parameters analyzed and soil electrical conductivity. In the second chapter of the thesis, the objective was to analyze the use of different silicon sources in sugarcane production in the state of Louisiana, United States of America. The experiment was conducted at the Louisiana State University Agricultural Research Station. Fertilization treatments consisted of the application of 0 kg·ha⁻¹ for the control, 2471 kg·ha⁻¹ for the limestone treatment, 2471 kg·ha⁻¹ for the sugarcane bagasse ash treatment, and 1235.5 and 2471 kg·ha⁻¹ for the silicate slag treatments. The experimental design used was a randomized block design, with plots consisting of three rows measuring 30.48 meters in length, using the sugarcane cultivar L01-299. Soil and leaf samples were collected 30 days after fertilization and again at harvest. In the laboratory, soil electrical conductivity analyses and colorimetric determinations were performed to quantify silicon in both soil and plant tissue. It was observed that soil electrical conductivity values were higher 30 days after fertilizer application compared to those measured at harvest time. The soil silicon analyses showed similar results between the two sampling periods, with no significant differences among the fertilization treatments. The treatments evaluated 30 days after fertilization exhibited higher silicon concentrations in the plants compared to those measured at harvest. In conclusion, soil silicon levels remained relatively consistent across the different sampling times; however, the highest concentration, 182.28 mg·kg⁻¹, was obtained 30 days after fertilization in the treatment with 2471 kg·ha⁻¹ of silicate. Regarding silicon content in the plant, the highest level (2.50 mg·kg⁻¹) was observed in the limestone treatment 30 days after application, while a lower value (0.86 mg·kg⁻¹) was recorded for the same treatment at harvest. Overall, it can be concluded that sustainable management practices combined with the use of technological tools lead to increased productivity and profitability for producers, while also ensuring the preservation of a clean and balanced environment for future generations. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Tese

2025-09-04T00:00:00Z Silva, Eric Valero Carvalho da https://tede.unioeste.br/handle/tede/8665 2026-09-22T18:51:40Z 2026-08-24T00:00:00Z

Title: Viabilidade técnico-econômica de sistemas fotovoltaicos na indústria leiteira: uma análise comparativa de topologias on-grid, off-grid, híbrido com bateria e zero grid Autor: Silva, Eric Valero Carvalho da Primeiro orientador: Santos, Reginaldo Ferreira Abstract: The growing need for sustainable technologies and the rise in energy costs impose significant challenges on agribusiness, especially on the dairy industry, an intensive consumer of electricity. In this context, distributed photovoltaic generation emerges as a strategic solution to increase the resilience and economic competitiveness of these properties. This dissertation aims to perform a technical and economic feasibility analysis of the implementation of four distinct photovoltaic system topologies—on-grid, off-grid, hybrid with battery storage, and zero-grid—in two dairy industries with different consumption and grid connection profiles.The analyses in this study comprise a Group A consumer unit (high voltage), located in Céu Azul, PR, and a Group B consumer unit (low voltage), in Engenheiro Coelho, SP. The sizing of the systems was based on the historical electricity consumption of the last 12 months for each unit, as well as on the average and minimum solar irradiation data for each location, ensuring an analysis appropriate to the operational reality and generation potential of each plant. From the results obtained, it was found that the on grid and zero grid systems are viable in both consumer units (UC-A and UC-B). On the other hand, the off grid and hybrid configurations showed to be financially unviable for the same scenarios. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Tese

2026-08-24T00:00:00Z Ponte, Enerdan Fernando Dal https://tede.unioeste.br/handle/tede/8661 2026-09-17T20:05:59Z 2026-07-17T00:00:00Z

Title: Automação Para Uma Usina Solar Tipo Tracker de Um Eixo Autor: Ponte, Enerdan Fernando Dal Primeiro orientador: Santos, Reginaldo Ferreira Abstract: Electricity generation via photovoltaic systems which harness incident solar radiation on solar cells to produce electricity is expanding in Brazil. This growth is largely due to the country's location in the intertropical zone, where solar irradiation levels exceed those of European nations such as Spain, Germany, and France. The use of photovoltaic generation systems is also increasing in agricultural areas; many producers are shifting toward net metering or selling electricity to utility companies by installing solar power plants on their properties. However, electricity generation efficiency is limited by the capacity of individual photovoltaic modules, as the installations are fixed while the sun’s position shifts throughout the day. This study developed an automated tracking system specifically a single-axis Tracker to follow the sun's position. A comparison was conducted between the fixed system and the operating Tracker system; the results showed a cumulative generation advantage of R$ 296,091.43 over a 25-year period in favor of the Tracker-based plant. Based on these findings, the Levelized Cost of Energy (LCOE) was determined to be R$ 361.07, and the discounted payback period for the investment was calculated at six years. Publisher: Universidade Estadual do Oeste do Paraná Tipo do documento: Tese

2026-07-17T00:00:00Z